Hotspot

rpl32-trnL-UAG

SSC · 118157-119340 · 1184 bp

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Pi 0.00676
MarkerSeek score 63.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1184
variable_sites
15
pi
0.00676
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGGCGTTGAAAGCTTTTTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1215-1299 bp 1.0 83.6
rpl32-trnL-UAG_p2 CGGCGTTGAAAGCTTTTTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1216-1300 bp 1.0 83.6
rpl32-trnL-UAG_p3 AAAGAAAGGATATCGGGCGG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.7 58.0 1232-1316 bp 1.0 82.8
rpl32-trnL-UAG_p4 AAAGAAAGGATATCGGGCGG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.7 58.0 1233-1317 bp 1.0 82.8
rpl32-trnL-UAG_p5 CGGCGTTGAAAGCTTTTTCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 1225-1309 bp 1.0 82.6