Hotspot

ndhF-rpl32

SSC · 117094-117991 · 898 bp

Back to Sida

Pi 0.00691
MarkerSeek score 62.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
898
variable_sites
12
pi
0.00691
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGGAAGTGGCACAAAAGGTA TGAAAAAGCTTTCAACGCCG 50.0 45.0 58.0 58.2 1048-1067 bp 1.0 80.2
ndhF-rpl32_p2 TGTTGCCGTCGGAAAAAGTA TGAAAAAGCTTTCAACGCCG 45.0 45.0 58.0 58.2 1096-1115 bp 1.0 79.8
ndhF-rpl32_p3 GGGAAGTGGCACAAAAGGTA CCGCCCGATATCCTTTCTTT 50.0 50.0 58.0 57.7 1031-1050 bp 1.0 79.5
ndhF-rpl32_p4 AAATAGGAACGGGAAGTGGC TGAAAAAGCTTTCAACGCCG 50.0 45.0 57.9 58.2 1058-1077 bp 1.0 79.3
ndhF-rpl32_p5 TGTTGCCGTCGGAAAAAGTA CCGCCCGATATCCTTTCTTT 45.0 50.0 58.0 57.7 1079-1098 bp 1.0 79.1