Hotspot

petD-rpoA

LSC · 82685-82921 · 237 bp

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Pi 0.038
MarkerSeek score 65.70
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
237
variable_sites
18
pi
0.038
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 TAATGGTTTCAGTACCCGCG CAGAATTGCCTCCCAGGATC 50.0 55.0 58.0 58.0 577-578 bp 1.0 82.5
petD-rpoA_p2 TAATGGTTTCAGTACCCGCG CGCCAAAGAACTTTCACGAC 50.0 50.0 58.0 58.0 925-926 bp 1.0 81.6
petD-rpoA_p3 TAATGGTTTCAGTACCCGCG GCCAAAGAACTTTCACGACG 50.0 50.0 58.0 58.0 924-925 bp 1.0 81.6
petD-rpoA_p4 GATTGGTACTGCAGTAGCCC CGCCAAAGAACTTTCACGAC 55.0 50.0 58.0 58.0 822-823 bp 1.0 81.5
petD-rpoA_p5 GATTGGTACTGCAGTAGCCC GCCAAAGAACTTTCACGACG 55.0 50.0 58.0 58.0 821-822 bp 1.0 81.5