Hotspot

psaJ-rpl33

LSC · 71589-72106 · 518 bp

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Pi 0.0134
MarkerSeek score 63.10
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
518
variable_sites
11
pi
0.0134
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ATCGTTTCTTCCCAGATGCG CACCGTTTCGAACACAACTG 50.0 50.0 58.3 57.9 621-632 bp 1.0 82.0
psaJ-rpl33_p2 ATCGTTTCTTCCCAGATGCG ATACCTGGAAATGCCCGTTG 50.0 50.0 58.3 58.2 653-664 bp 1.0 80.7
psaJ-rpl33_p3 ATCGTTTCTTCCCAGATGCG ACACCGTTTCGAACACAACT 50.0 45.0 58.3 58.3 622-633 bp 1.0 80.5
psaJ-rpl33_p4 ATCGTTTCTTCCCAGATGCG GGAAATGCCCGTTGATTCCT 50.0 50.0 58.3 58.5 647-658 bp 1.0 78.4
psaJ-rpl33_p5 ATCGTTTCTTCCCAGATGCG AACACCGTTTCGAACACAAC 50.0 45.0 58.3 57.5 623-634 bp 1.0 78.4