Hotspot

psbE-petL

LSC · 69313-70205 · 893 bp

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Pi 0.0122
MarkerSeek score 67.80
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
893
variable_sites
21
pi
0.0122
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AATAACCAACCCGCGATGAA AGCCGAGGTTATAGTTAAAGCC 45.0 45.5 57.9 57.9 1058-1072 bp 1.0 64.5
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC AGCCGAGGTTATAGTTAAAGCC 50.0 45.5 57.8 57.9 975-989 bp 1.0 64.4
psbE-petL_p3 AATAACCAACCCGCGATGAA CGAGGTTATAGTTAAAGCCGC 45.0 47.6 57.9 56.9 1055-1069 bp 1.0 64.1
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC AGCCGAGGTTATAGTTAAAGCC 55.0 45.5 58.2 57.9 970-984 bp 1.0 64.1
psbE-petL_p5 TGCTGACGAATAACCAACCC AGCCGAGGTTATAGTTAAAGCC 50.0 45.5 58.2 57.9 1066-1080 bp 1.0 64.0