Hotspot

rps4-trnT-UGU

LSC · 48288-48791 · 504 bp

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Pi 0.0201
MarkerSeek score 74.80
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
504
variable_sites
17
pi
0.0201
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TAAGATCGCTTCCGGCTCTA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 645-675 bp 1.0 84.1
rps4-trnT-UGU_p2 TTTCTAAGATCGCTTCCGGC CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 649-679 bp 1.0 83.7
rps4-trnT-UGU_p3 TAAGATCGCTTCCGGCTCTA ATGGTCATCGGTTCGATTCC 50.0 50.0 58.0 57.8 613-643 bp 1.0 83.5
rps4-trnT-UGU_p4 TTTCTAAGATCGCTTCCGGC ATGGTCATCGGTTCGATTCC 50.0 50.0 58.1 57.8 617-647 bp 1.0 83.1
rps4-trnT-UGU_p5 TAAGATCGCTTCCGGCTCTA CCGCTTAGCTCAGAGGTTAG 50.0 55.0 58.0 57.5 653-683 bp 1.0 81.1