Hotspot

psaA-ycf3

LSC · 43931-44918 · 988 bp

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Pi 0.00702
MarkerSeek score 67.20
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
988
variable_sites
13
pi
0.00702
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT TGGTTAAAGATCACGAGGCG 50.0 50.0 57.9 58.0 1098-1151 bp 1.0 82.4
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT GGTTAAAGATCACGAGGCGT 50.0 50.0 57.9 58.0 1097-1150 bp 1.0 82.4
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 1166-1219 bp 1.0 81.9
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT GCTGAGTATTGGAGACAGGC 50.0 55.0 57.9 58.1 1158-1211 bp 1.0 81.8
psaA-ycf3_p5 TTCTTGAGAAATGACCCGGC TGGTTAAAGATCACGAGGCG 50.0 50.0 58.2 58.0 1121-1174 bp 1.0 81.8