Hotspot

psbZ-trnG-GCC

LSC · 37824-38561 · 738 bp

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Pi 0.021
MarkerSeek score 68.10
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
738
variable_sites
28
pi
0.021
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 883-930 bp 1.0 83.2
psbZ-trnG-GCC_p2 GCTTCTCCTGATGGTTGGTT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 881-928 bp 1.0 83.2
psbZ-trnG-GCC_p3 CTCCTGATGGTTGGTTGAGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.1 57.8 877-924 bp 1.0 77.9
psbZ-trnG-GCC_p4 TGCTTCTCCTGATGGTTGGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.9 57.8 882-929 bp 1.0 77.4
psbZ-trnG-GCC_p5 TCTCCTGATGGTTGGTTGAG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 56.8 57.8 878-925 bp 1.0 75.6