Hotspot

petN-psbM

LSC · 29632-30728 · 1097 bp

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Pi 0.00367
MarkerSeek score 61.90
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1097
variable_sites
8
pi
0.00367
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA ATTGCTACAGCGCTGTTCAT 45.0 45.0 58.0 58.3 1254-1291 bp 1.0 78.9
petN-psbM_p2 TCTTGCTTGGGCTGCTTTAA TATTGCTACAGCGCTGTTCA 45.0 45.0 58.0 57.6 1255-1292 bp 1.0 77.9
petN-psbM_p3 AGTAAGTCTTGCTTGGGCTG ATTGCTACAGCGCTGTTCAT 50.0 45.0 57.8 58.3 1260-1297 bp 1.0 77.4
petN-psbM_p4 TCTTGCTTGGGCTGCTTTAA TTGCTACAGCGCTGTTCATT 45.0 45.0 58.0 58.5 1253-1290 bp 1.0 77.1
petN-psbM_p5 AGTAAGTCTTGCTTGGGCTG TATTGCTACAGCGCTGTTCA 50.0 45.0 57.8 57.6 1261-1298 bp 1.0 76.4