Hotspot

trnS-GCU-trnG-GCC

LSC · 8392-9188 · 797 bp

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Pi 0.0121
MarkerSeek score 64.30
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
797
variable_sites
19
pi
0.0121
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA GAATCAAACCGAGAGACCCC 50.0 55.0 58.0 58.0 891-910 bp 1.0 83.5
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC GAATCAAACCGAGAGACCCC 50.0 55.0 58.0 58.0 890-909 bp 1.0 83.5
trnS-GCU-trnG-GCC_p3 CGCACAACGGATTAGCAATC GAATCAAACCGAGAGACCCC 50.0 55.0 57.9 58.0 906-925 bp 1.0 82.7
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA CCGAGAGACCCCTTAACTCT 50.0 55.0 58.0 57.9 883-902 bp 1.0 82.6
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC CCGAGAGACCCCTTAACTCT 50.0 55.0 58.0 57.9 882-901 bp 1.0 82.6