Hotspot

ycf1

SSC · 124356-129890 · 5535 bp

Pi 0.346

MarkerSeek score 75.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5535
variable_sites: 1663
pi: 0.346
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGAGCCTTGGATCATTCGAT	AAGAGCAGAAGCCTGGGATA	50.0	50.0	57.8	58.1	721 bp	0.5	57.8
ycf1_p2	CGAGCCTTGGATCATTCGAT	TCCTGAATGGTCCGAGGATT	50.0	50.0	57.8	58.1	577 bp	0.5	57.8
ycf1_p3	CGAGCCTTGGATCATTCGAT	TTCCTGAATGGTCCGAGGAT	50.0	50.0	57.8	58.1	578 bp	0.5	57.8
ycf1_p4	ATACTGGACGAGCCTTGGAT	AAGAGCAGAAGCCTGGGATA	50.0	50.0	58.2	58.1	729 bp	0.5	57.4
ycf1_p5	ATACTGGACGAGCCTTGGAT	TCCTGAATGGTCCGAGGATT	50.0	50.0	58.2	58.1	585 bp	0.5	57.4