Hotspot

petD

LSC · 76488-77710 · 1223 bp

Back to Sicyos

Pi 0.153
MarkerSeek score 82.00
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1223
variable_sites
167
pi
0.153
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 TATCGTCCGACCGTTACTGA CACTTCATGAAGCCTCTCGG 50.0 55.0 58.0 58.4 2449-2464 bp 1.0 87.2
petD_p2 ACTATCGTCCGACCGTTACT CACTTCATGAAGCCTCTCGG 50.0 55.0 58.0 58.4 2451-2466 bp 1.0 87.2
petD_p3 GCGTGTATCTTACTGGTGGG CACTTCATGAAGCCTCTCGG 55.0 55.0 58.1 58.4 2313-2328 bp 1.0 87.2
petD_p4 TATCGTCCGACCGTTACTGA AGCACTTCATGAAGCCTCTC 50.0 50.0 58.0 57.6 2451-2466 bp 1.0 86.8
petD_p5 ACTATCGTCCGACCGTTACT AGCACTTCATGAAGCCTCTC 50.0 50.0 58.0 57.6 2453-2468 bp 1.0 86.8