Hotspot

ndhI

SSC · 119626-120135 · 510 bp

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Pi 0.503
MarkerSeek score 79.30
Primer available yes
Label ndhI

Feature Metadata

label_name
ndhI
feature_type
gene
region
SSC
length_bp
510
variable_sites
221
pi
0.503
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI_p1 AAACTGGGCGTTTGCTAGAA AAATAATCCCGACACGCTCC 45.0 50.0 58.0 58.1 1726 bp 0.5 59.4
ndhI_p2 CATGTATCGGTCCAGGCAAA AAATAATCCCGACACGCTCC 50.0 50.0 58.0 58.1 1580 bp 0.5 59.3
ndhI_p3 CAAGAACCAGTCCCAACGAA AAATAATCCCGACACGCTCC 50.0 50.0 58.0 58.1 1685 bp 0.5 59.3
ndhI_p4 CGGTCCAGGCAAATCCATTA AAATAATCCCGACACGCTCC 50.0 50.0 57.9 58.1 1573 bp 0.5 59.2
ndhI_p5 AAACTGGGCGTTTGCTAGAA TGCGAGCGATTACCTTTTGA 45.0 45.0 58.0 57.9 1484 bp 0.5 59.1