Hotspot

ndhH

SSC · 122410-123591 · 1182 bp

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Pi 0.481
MarkerSeek score 81.90
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
SSC
length_bp
1182
variable_sites
506
pi
0.481
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 ATCCGTTGTTGTATCCCTGC CGGAAAACGCCAACGATTAC 50.0 50.0 58.0 58.1 1579 bp 0.5 58.7
ndhH_p2 GATGGGAGCCACACTTGAAA CGGAAAACGCCAACGATTAC 50.0 50.0 58.1 58.1 1823 bp 0.5 58.3
ndhH_p3 TCAGGTCCAATCCGTTGTTG CGGAAAACGCCAACGATTAC 50.0 50.0 58.1 58.1 1588 bp 0.5 58.1
ndhH_p4 ATCCGTTGTTGTATCCCTGC TCGGAAAACGCCAACGATTA 50.0 45.0 58.0 58.2 1580 bp 0.5 58.0
ndhH_p5 GATGGGAGCCACACTTGAAA TCGGAAAACGCCAACGATTA 50.0 45.0 58.1 58.2 1824 bp 0.5 57.6