Hotspot

ndhG

SSC · 118790-119320 · 531 bp

Back to Sicyos

Pi 0.475
MarkerSeek score 78.30
Primer available yes
Label ndhG

Feature Metadata

label_name
ndhG
feature_type
gene
region
SSC
length_bp
531
variable_sites
205
pi
0.475
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG_p1 ATTCATGCGATAGGGCCAAG CCCGGTCAGATCAATAAGGC 50.0 55.0 58.1 58.1 1240 bp 0.5 58.3
ndhG_p2 ATTCATGCGATAGGGCCAAG CCGGTCAGATCAATAAGGCC 50.0 55.0 58.1 58.1 1239 bp 0.5 58.3
ndhG_p3 ATTGAAGGGGGATGTGTGTG CCCGGTCAGATCAATAAGGC 50.0 55.0 57.8 58.1 660 bp 0.5 57.3
ndhG_p4 ATTGAAGGGGGATGTGTGTG CCGGTCAGATCAATAAGGCC 50.0 55.0 57.8 58.1 659 bp 0.5 57.3
ndhG_p5 TGCGATAGGGCCAAGATACT CCGGTCAGATCAATAAGGCC 50.0 55.0 58.3 58.1 1234 bp 0.5 56.8