Hotspot

ndhF-rpl32

SSC · 112882-113475 · 594 bp

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Pi 0.432
MarkerSeek score 80.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
594
variable_sites
214
pi
0.432
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTTTTGGTTGCCGTTGGAA GGCGATGGGGATTCGTATTT 45.0 50.0 58.0 58.1 1257 bp 0.5 59.0
ndhF-rpl32_p2 AAAAGACCACACACGTCGAA GGCGATGGGGATTCGTATTT 45.0 50.0 58.0 58.1 1278 bp 0.5 59.0
ndhF-rpl32_p3 AAGGTTTTTGGTTGCCGTTG GGCGATGGGGATTCGTATTT 45.0 50.0 58.0 58.1 1260 bp 0.5 59.0
ndhF-rpl32_p4 CGGCGCACAAAGTAACAAAT GGCGATGGGGATTCGTATTT 45.0 50.0 58.0 58.1 1843 bp 0.5 58.8
ndhF-rpl32_p5 ACTCGGAAAAGACCACACAC GGCGATGGGGATTCGTATTT 50.0 50.0 58.1 58.1 1284 bp 0.5 58.7