primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	trnK-rps16	1	AGTCGCGGTCTTACAAACTC	AGAAGGCAGGGGTTTTTACG	20	20	50.0	50.0	57.9	58.1	6.1	0.0	0.156102	4173	4972	958-1024	1.000000	85.5
trnK-rps16_p2	trnK-rps16	2	GTCGCGGTCTTACAAACTCT	AGAAGGCAGGGGTTTTTACG	20	20	50.0	50.0	57.9	58.1	6.1	0.0	0.156102	4173	4972	957-1023	1.000000	85.5
trnK-rps16_p3	trnK-rps16	3	AGTCGCGGTCTTACAAACTC	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	57.9	58.2	6.1	1.3	0.252056	4173	4972	995-1061	1.000000	84.4
trnK-rps16_p4	trnK-rps16	4	GTCGCGGTCTTACAAACTCT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	57.9	58.2	6.1	1.3	0.252056	4173	4972	994-1060	1.000000	84.4
trnK-rps16_p5	trnK-rps16	5	CAGTCGCGGTCTTACAAACT	AGAAGGCAGGGGTTTTTACG	20	20	50.0	50.0	58.2	58.1	6.1	0.0	0.314793	4173	4972	959-1025	1.000000	84.2
petN-psbM_p1	petN-psbM	1	ATGGGGAAGAAGTGGACTCT	ACGTAAAAACAATCAGTCAAAGTGA	20	25	50.0	32.0	57.6	57.9	0.0	0.0	5.430044	29478	30102	665-676	1.000000	46.2
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	ACGTAAAAACAATCAGTCAAAGTG	20	24	50.0	33.3	57.6	56.7	0.0	0.0	5.697166	29478	30102	665-676	1.000000	46.2
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	CGTAAAAACAATCAGTCAAAGTGA	20	24	50.0	33.3	57.6	56.4	0.0	0.0	5.935048	29478	30102	664-675	1.000000	46.2
petN-psbM_p4	petN-psbM	4	ATGGGGAAGAAGTGGACTCT	ACGTAAAAACAATCAGTCAAAGT	20	23	50.0	30.4	57.6	55.3	0.0	0.0	6.043754	29478	30102	665-676	1.000000	46.2
petN-psbM_p5	petN-psbM	5	CTCGCTTGGGCTGCTTTAAT	ACGTAAAAACAATCAGTCAAAGTGA	20	25	50.0	32.0	58.9	57.9	0.0	0.0	5.981132	29478	30102	718-729	1.000000	45.7
petG-trnW-CCA_p1	petG-trnW-CCA	1	GGGGATCAGTTGGACCTTTG	CAAAACCCGATGTCGTAGGT	20	20	55.0	50.0	58.2	57.9	0.0	1.4	0.249526	66256	66371	173-176	1.000000	84.4
petG-trnW-CCA_p2	petG-trnW-CCA	2	GGGGATCAGTTGGACCTTTG	CCCGATGTCGTAGGTTCAAA	20	20	55.0	50.0	58.2	57.6	0.0	1.4	0.534117	66256	66371	168-171	1.000000	82.3
petG-trnW-CCA_p3	petG-trnW-CCA	3	GGGGATCAGTTGGACCTTTG	TCCAAAACCCGATGTCGTAG	20	20	55.0	50.0	58.2	57.6	0.0	1.4	0.534117	66256	66371	175-178	1.000000	82.1
petG-trnW-CCA_p4	petG-trnW-CCA	4	GGGGATCAGTTGGACCTTTG	ACGCTCTTAGTTCAGTTCGG	20	20	55.0	50.0	58.2	57.7	0.0	0.0	0.509874	66256	66371	208-211	1.000000	81.3
petG-trnW-CCA_p5	petG-trnW-CCA	5	GGGGATCAGTTGGACCTTTG	CGCTCTTAGTTCAGTTCGGT	20	20	55.0	50.0	58.2	57.7	0.0	0.0	0.509874	66256	66371	207-210	1.000000	81.3
petD_p1	petD	1	TATCGTCCGACCGTTACTGA	CACTTCATGAAGCCTCTCGG	20	20	50.0	55.0	58.0	58.4	0.0	31.0	0.373101	76488	77710	2449-2464	1.000000	87.2
petD_p2	petD	2	ACTATCGTCCGACCGTTACT	CACTTCATGAAGCCTCTCGG	20	20	50.0	55.0	58.0	58.4	0.0	31.0	0.380175	76488	77710	2451-2466	1.000000	87.2
petD_p3	petD	3	GCGTGTATCTTACTGGTGGG	CACTTCATGAAGCCTCTCGG	20	20	55.0	55.0	58.1	58.4	0.0	31.0	0.414404	76488	77710	2313-2328	1.000000	87.2
petD_p4	petD	4	TATCGTCCGACCGTTACTGA	AGCACTTCATGAAGCCTCTC	20	20	50.0	50.0	58.0	57.6	0.0	31.0	0.422681	76488	77710	2451-2466	1.000000	86.8
petD_p5	petD	5	ACTATCGTCCGACCGTTACT	AGCACTTCATGAAGCCTCTC	20	20	50.0	50.0	58.0	57.6	0.0	31.0	0.429755	76488	77710	2453-2468	1.000000	86.8
rpl16_p1	rpl16	1	ACACATCAATTCTCGGGCTC	GGTGTTGAAGGAATTGCACG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.080315	81214	82690	2321-2321	1.000000	90.0
rpl16_p2	rpl16	2	ACACATCAATTCTCGGGCTC	AAACCCGTATGGACACCCTA	20	20	50.0	50.0	58.0	58.0	0.0	1.3	0.074387	81214	82690	2133-2133	0.500000	59.4
rpl16_p3	rpl16	3	ACACATCAATTCTCGGGCTC	AACCCGTATGGACACCCTAA	20	20	50.0	50.0	58.0	58.0	0.0	1.3	0.074387	81214	82690	2132-2132	0.500000	59.4
rpl16_p4	rpl16	4	TCTCAATTCTCGGGCAATCG	AAACCCGTATGGACACCCTA	20	20	50.0	50.0	58.1	58.0	0.0	1.3	0.104575	81214	82690	2391-2391	0.500000	59.2
rpl16_p5	rpl16	5	TCTCAATTCTCGGGCAATCG	AACCCGTATGGACACCCTAA	20	20	50.0	50.0	58.1	58.0	0.0	1.3	0.104575	81214	82690	2390-2390	0.500000	59.2
rpl2-rpl23_p1	rpl2-rpl23	1	CCACAACGATGCTGTCCATA	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	58.0	58.3	0.0	13.0	0.288602	85910	85927	181-181	1.000000	78.1
rpl2-rpl23_p2	rpl2-rpl23	2	GACTGTCTACGGCTCCATTG	CGCTTCAACCGGGTTATTCT	20	20	55.0	50.0	58.1	58.3	0.0	13.0	0.337942	85910	85927	129-129	1.000000	77.9
rpl2-rpl23_p3	rpl2-rpl23	3	CCACAACGATGCTGTCCATA	ATCATTACGCTTCAACCGGG	20	20	50.0	50.0	58.0	58.3	0.0	6.3	0.359070	85910	85927	188-188	1.000000	77.6
rpl2-rpl23_p4	rpl2-rpl23	4	GACTGTCTACGGCTCCATTG	ATCATTACGCTTCAACCGGG	20	20	55.0	50.0	58.1	58.3	0.0	6.3	0.408411	85910	85927	136-136	1.000000	77.3
rpl2-rpl23_p5	rpl2-rpl23	5	TGCTCGGGGTAGAAGTTTTG	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	57.8	58.3	0.0	13.0	0.444749	85910	85927	105-105	1.000000	77.2
ndhF-rpl32_p1	ndhF-rpl32	1	GTTTTTGGTTGCCGTTGGAA	GGCGATGGGGATTCGTATTT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.119331	112882	113475	1257-1257	0.500000	59.0
ndhF-rpl32_p2	ndhF-rpl32	2	AAAAGACCACACACGTCGAA	GGCGATGGGGATTCGTATTT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.120727	112882	113475	1278-1278	0.500000	59.0
ndhF-rpl32_p3	ndhF-rpl32	3	AAGGTTTTTGGTTGCCGTTG	GGCGATGGGGATTCGTATTT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.124928	112882	113475	1260-1260	0.500000	59.0
ndhF-rpl32_p4	ndhF-rpl32	4	CGGCGCACAAAGTAACAAAT	GGCGATGGGGATTCGTATTT	20	20	45.0	50.0	58.0	58.1	10.8	0.0	0.152678	112882	113475	1843-1843	0.500000	58.8
ndhF-rpl32_p5	ndhF-rpl32	5	ACTCGGAAAAGACCACACAC	GGCGATGGGGATTCGTATTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.158325	112882	113475	1284-1284	0.500000	58.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCAGCGTTGAAAGCTTTTT	GGAGAGCGGGGACTAATAGT	20	20	45.0	55.0	58.4	58.0	14.0	0.0	0.430965	113638	114728	1758-1758	0.500000	56.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCAGCGTTGAAAGCTTTTT	TGGAGAGCGGGGACTAATAG	20	20	45.0	55.0	58.4	58.0	14.0	0.0	0.432389	113638	114728	1759-1759	0.500000	56.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCAGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.4	58.0	14.0	0.0	0.437017	113638	114728	1205-1205	0.500000	56.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGCAGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.4	58.0	14.0	0.0	0.438388	113638	114728	1206-1206	0.500000	56.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGCAGCGTTGAAAGCTTTTT	CGGGGACTAATAGTGGGGAT	20	20	45.0	55.0	58.4	58.0	14.0	0.0	0.454823	113638	114728	1752-1752	0.500000	56.4
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	GGTATGAGCGGTTTTGTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030642	114911	115882	1732-1732	0.500000	59.8
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	GGTATGAGCGGTTTTGTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032012	114911	115882	1731-1731	0.500000	59.7
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	TTATCGATGGCTTCCCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	8.5	0.040512	114911	115882	1759-1759	0.500000	59.7
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	TTATCGATGGCTTCCCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	8.5	0.041882	114911	115882	1758-1758	0.500000	59.7
ccsA_p5	ccsA	5	GTGAAATCGGTAGACACGCT	CATGTTTGTGTTCGTCAGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044387	114911	115882	1181-1181	0.500000	59.6
ndhD_p1	ndhD	1	GCCTGACGAACACAAACATG	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.055300	116134	117636	1901-1901	0.500000	59.6
ndhD_p2	ndhD	2	GCCTGACGAACACAAACATG	GGCATGAAACAACTCGAAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.067453	116134	117636	1907-1907	0.500000	59.5
ndhD_p3	ndhD	3	GCCTGACGAACACAAACATG	TGAATCTGCTTGTCCGACAG	20	20	50.0	50.0	58.0	57.9	0.0	0.6	0.121166	116134	117636	1954-1954	0.500000	59.0
ndhD_p4	ndhD	4	GCCTGACGAACACAAACATG	CTTGGCCCTATCGCATGAAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.134966	116134	117636	2263-2263	0.500000	58.9
ndhD_p5	ndhD	5	ATTGAAGAAAGGGCCTGACG	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	58.2	58.0	6.9	0.0	0.192359	116134	117636	1913-1913	0.500000	58.5
psaC-ndhE_p1	psaC-ndhE	1	ACCCATGCTTCGAGTTGTTT	CACACACATCCCCCTTCAAT	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.239396	118014	118258	962-962	0.500000	58.1
psaC-ndhE_p2	psaC-ndhE	2	GCTTCGAGTTGTTTCATGCC	CACACACATCCCCCTTCAAT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.251549	118014	118258	956-956	0.500000	58.0
psaC-ndhE_p3	psaC-ndhE	3	CTGTCGGACAAGCAGATTCA	CACACACATCCCCCTTCAAT	20	20	50.0	50.0	57.9	57.8	12.5	0.0	0.305262	118014	118258	909-909	0.500000	57.6
psaC-ndhE_p4	psaC-ndhE	4	CTGGCCAAGCTGCTAAAGTA	CACACACATCCCCCTTCAAT	20	20	50.0	50.0	57.9	57.8	7.0	0.0	0.334513	118014	118258	1424-1424	0.500000	57.3
psaC-ndhE_p5	psaC-ndhE	5	TGGCCAAGCTGCTAAAGTAG	CACACACATCCCCCTTCAAT	20	20	50.0	50.0	57.9	57.8	0.6	0.0	0.334513	118014	118258	1423-1423	0.500000	57.3
ndhG_p1	ndhG	1	ATTCATGCGATAGGGCCAAG	CCCGGTCAGATCAATAAGGC	20	20	50.0	55.0	58.1	58.1	6.2	0.0	0.214174	118790	119320	1240-1240	0.500000	58.3
ndhG_p2	ndhG	2	ATTCATGCGATAGGGCCAAG	CCGGTCAGATCAATAAGGCC	20	20	50.0	55.0	58.1	58.1	6.2	0.0	0.214174	118790	119320	1239-1239	0.500000	58.3
ndhG_p3	ndhG	3	ATTGAAGGGGGATGTGTGTG	CCCGGTCAGATCAATAAGGC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.336417	118790	119320	660-660	0.500000	57.3
ndhG_p4	ndhG	4	ATTGAAGGGGGATGTGTGTG	CCGGTCAGATCAATAAGGCC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.336417	118790	119320	659-659	0.500000	57.3
ndhG_p5	ndhG	5	TGCGATAGGGCCAAGATACT	CCGGTCAGATCAATAAGGCC	20	20	50.0	55.0	58.3	58.1	6.2	0.0	0.398733	118790	119320	1234-1234	0.500000	56.8
ndhI_p1	ndhI	1	AAACTGGGCGTTTGCTAGAA	AAATAATCCCGACACGCTCC	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.074875	119626	120135	1726-1726	0.500000	59.4
ndhI_p2	ndhI	2	CATGTATCGGTCCAGGCAAA	AAATAATCCCGACACGCTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.087492	119626	120135	1580-1580	0.500000	59.3
ndhI_p3	ndhI	3	CAAGAACCAGTCCCAACGAA	AAATAATCCCGACACGCTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.088741	119626	120135	1685-1685	0.500000	59.3
ndhI_p4	ndhI	4	CGGTCCAGGCAAATCCATTA	AAATAATCCCGACACGCTCC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.101869	119626	120135	1573-1573	0.500000	59.2
ndhI_p5	ndhI	5	AAACTGGGCGTTTGCTAGAA	TGCGAGCGATTACCTTTTGA	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.111841	119626	120135	1484-1484	0.500000	59.1
ndhA_p1	ndhA	1	TGCAGCTCGTACTGTTTGTT	ATTGGGGGTTATCAGGACCA	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.057497	120217	122408	2867-2867	0.500000	59.5
ndhA_p2	ndhA	2	TGCAGCTCGTACTGTTTGTT	TGGGGGTTATCAGGACCAAT	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.057497	120217	122408	2865-2865	0.500000	59.5
ndhA_p3	ndhA	3	TTGCAGCTCGTACTGTTTGT	ATTGGGGGTTATCAGGACCA	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.057497	120217	122408	2868-2868	0.500000	59.5
ndhA_p4	ndhA	4	TTGCAGCTCGTACTGTTTGT	TGGGGGTTATCAGGACCAAT	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.057497	120217	122408	2866-2866	0.500000	59.5
ndhA_p5	ndhA	5	TGCAGCTCGTACTGTTTGTT	TAGGGGATCAGGGTGGTTTT	20	20	45.0	50.0	58.1	57.9	8.8	0.0	0.120727	120217	122408	2489-2489	0.500000	59.0
ndhH_p1	ndhH	1	ATCCGTTGTTGTATCCCTGC	CGGAAAACGCCAACGATTAC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.165092	122410	123591	1579-1579	0.500000	58.7
ndhH_p2	ndhH	2	GATGGGAGCCACACTTGAAA	CGGAAAACGCCAACGATTAC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.217005	122410	123591	1823-1823	0.500000	58.3
ndhH_p3	ndhH	3	TCAGGTCCAATCCGTTGTTG	CGGAAAACGCCAACGATTAC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.235829	122410	123591	1588-1588	0.500000	58.1
ndhH_p4	ndhH	4	ATCCGTTGTTGTATCCCTGC	TCGGAAAACGCCAACGATTA	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.245129	122410	123591	1580-1580	0.500000	58.0
ndhH_p5	ndhH	5	GATGGGAGCCACACTTGAAA	TCGGAAAACGCCAACGATTA	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.297042	122410	123591	1824-1824	0.500000	57.6
ycf1_p1	ycf1	1	CGAGCCTTGGATCATTCGAT	AAGAGCAGAAGCCTGGGATA	20	20	50.0	50.0	57.8	58.1	5.7	0.0	0.275773	127010	127129	721-721	0.500000	57.8
ycf1_p2	ycf1	2	CGAGCCTTGGATCATTCGAT	TCCTGAATGGTCCGAGGATT	20	20	50.0	50.0	57.8	58.1	5.7	0.0	0.281247	127010	127129	577-577	0.500000	57.8
ycf1_p3	ycf1	3	CGAGCCTTGGATCATTCGAT	TTCCTGAATGGTCCGAGGAT	20	20	50.0	50.0	57.8	58.1	5.7	0.0	0.281247	127010	127129	578-578	0.500000	57.8
ycf1_p4	ycf1	4	ATACTGGACGAGCCTTGGAT	AAGAGCAGAAGCCTGGGATA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.320237	127010	127129	729-729	0.500000	57.4
ycf1_p5	ycf1	5	ATACTGGACGAGCCTTGGAT	TCCTGAATGGTCCGAGGATT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.325711	127010	127129	585-585	0.500000	57.4