Hotspot

rps16

LSC · 5008-6151 · 1144 bp

Back to Sibbaldianthe

Pi 0.0119

MarkerSeek score 48.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1144
variable_sites: 21
pi: 0.0119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAACCGGGTTAGTCCTTTGT	TGGGTTCCAAACTGAAGGAA	45.0	45.0	57.0	56.9	1213-1241 bp	1.0	64.1
rps16_p2	AACCGGGTTAGTCCTTTGTT	TGGGTTCCAAACTGAAGGAA	45.0	45.0	57.0	56.9	1212-1240 bp	1.0	64.1
rps16_p3	ACCGGGTTAGTCCTTTGTTT	TGGGTTCCAAACTGAAGGAA	45.0	45.0	57.0	56.9	1211-1239 bp	1.0	64.1
rps16_p4	AACCGGGTTAGTCCTTTGTT	AGTACCAACCGAACCAATGA	45.0	45.0	57.0	56.8	1274-1302 bp	1.0	63.2
rps16_p5	ACCGGGTTAGTCCTTTGTTT	AGTACCAACCGAACCAATGA	45.0	45.0	57.0	56.8	1273-1301 bp	1.0	63.2