Hotspot

rpl32-trnL-UAG

SSC · 113788-114474 · 687 bp

Back to Sibbaldianthe

Pi 0.0206

MarkerSeek score 58.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 687
variable_sites: 22
pi: 0.0206
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGAAATCACTTTCTACCGG	AGCGTGTCTACCGATTTCAC	50.0	50.0	56.9	58.0	746-808 bp	1.0	77.5
rpl32-trnL-UAG_p2	GCGAAATCACTTTCTACCGG	CAGCGTGTCTACCGATTTCA	50.0	50.0	56.9	58.0	747-809 bp	1.0	77.5
rpl32-trnL-UAG_p3	GCGAAATCACTTTCTACCGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	56.9	57.9	756-818 bp	1.0	76.5
rpl32-trnL-UAG_p4	GCGAAATCACTTTCTACCGG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	56.9	57.8	755-817 bp	1.0	76.0
rpl32-trnL-UAG_p5	GCGAAATCACTTTCTACCGG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	56.9	57.8	754-816 bp	1.0	76.0