Hotspot

ndhF-rpl32

SSC · 113014-113628 · 615 bp

Back to Sibbaldianthe

Pi 0.0155

MarkerSeek score 62.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 615
variable_sites: 16
pi: 0.0155
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAACGGT	TTTTTGAACTTGAGGTGCGC	50.0	45.0	57.8	57.8	695-704 bp	1.0	80.2
ndhF-rpl32_p2	AAAGTAGAAGCCCTGCTCCT	TTTTTGAACTTGAGGTGCGC	50.0	45.0	58.3	57.8	728-737 bp	1.0	78.8
ndhF-rpl32_p3	CTGGAAGTGGAATGAACGGT	GAACTTGAGGTGCGCTTTTT	50.0	45.0	57.8	57.5	690-699 bp	1.0	77.9
ndhF-rpl32_p4	AAAAGTAGAAGCCCTGCTCC	TTTTTGAACTTGAGGTGCGC	50.0	45.0	57.5	57.8	729-738 bp	1.0	77.6
ndhF-rpl32_p5	AAGTAGAAGCCCTGCTCCTA	TTTTTGAACTTGAGGTGCGC	50.0	45.0	57.4	57.8	727-736 bp	1.0	77.1