Hotspot

rpl36-rps8

LSC · 79996-80503 · 508 bp

Back to Sibbaldianthe

Pi 0.00886

MarkerSeek score 53.00

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 508
variable_sites: 7
pi: 0.00886
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CCTACGGATCAAGCGACATT	GAATCGGCGGGGAAATTTTG	50.0	50.0	57.8	58.1	583-600 bp	1.0	81.1
rpl36-rps8_p2	CGAACGGAGGCCCTTATTTT	GAATCGGCGGGGAAATTTTG	50.0	50.0	58.3	58.1	549-566 bp	1.0	81.1
rpl36-rps8_p3	ACTATAATTCGTCCCCGCCT	GAATCGGCGGGGAAATTTTG	50.0	50.0	58.3	58.1	600-617 bp	1.0	80.5
rpl36-rps8_p4	CCTACGGATCAAGCGACATT	GAATAGAAGGAATCGGCGGG	50.0	55.0	57.8	58.2	592-609 bp	1.0	80.0
rpl36-rps8_p5	CGAACGGAGGCCCTTATTTT	GAATAGAAGGAATCGGCGGG	50.0	55.0	58.3	58.2	558-575 bp	1.0	80.0