Hotspot

rpl20-rps12

LSC · 69312-70083 · 772 bp

Back to Sibbaldianthe

Pi 0.00584
MarkerSeek score 63.30
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
772
variable_sites
7
pi
0.00584
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTTGAATGAGCCCCTCGAAA TGCAAGACAGCCAATCAGAA 50.0 45.0 57.9 57.7 941-946 bp 1.0 78.0
rpl20-rps12_p2 TGAATGAGCCCCTCGAAAAG TGCAAGACAGCCAATCAGAA 50.0 45.0 57.9 57.7 939-944 bp 1.0 78.0
rpl20-rps12_p3 GAATGAGCCCCTCGAAAAGT TGCAAGACAGCCAATCAGAA 50.0 45.0 57.9 57.7 938-943 bp 1.0 78.0
rpl20-rps12_p4 GAGCCCCTCGAAAAGTTGAT TGCAAGACAGCCAATCAGAA 50.0 45.0 57.9 57.7 934-939 bp 1.0 78.0
rpl20-rps12_p5 AGCCCCTCGAAAAGTTGATG TGCAAGACAGCCAATCAGAA 50.0 45.0 58.2 57.7 933-938 bp 1.0 77.5