Hotspot

trnP-UGG-psaJ

LSC · 66881-67408 · 528 bp

Back to Sibbaldianthe

Pi 0.0165

MarkerSeek score 65.20

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 528
variable_sites: 11
pi: 0.0165
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	GGGATGACAGGATTCGAACC	GGAATATCAACGCATCCGGA	55.0	50.0	58.0	57.8	677-718 bp	1.0	83.6
trnP-UGG-psaJ_p2	ATCTCCCCCGTTACTGTTCA	GGAATATCAACGCATCCGGA	50.0	50.0	58.1	57.8	715-756 bp	1.0	83.5
trnP-UGG-psaJ_p3	GGGATGACAGGATTCGAACC	AGTACTTACTACCGGTGCCA	55.0	50.0	58.0	57.8	607-648 bp	1.0	83.3
trnP-UGG-psaJ_p4	AATCGATCTCCCCCGTTACT	GGAATATCAACGCATCCGGA	50.0	50.0	57.9	57.8	720-761 bp	1.0	83.2
trnP-UGG-psaJ_p5	CCCCCGTTACTGTTCAGAAG	GGAATATCAACGCATCCGGA	55.0	50.0	57.9	57.8	711-752 bp	1.0	83.1