Hotspot

trnT-GGU-psbD

LSC · 31794-33194 · 1401 bp

Back to Sibbaldianthe

Pi 0.00476
MarkerSeek score 57.70
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1401
variable_sites
11
pi
0.00476
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA GGCCAGACCAACCTACAAAA 50.0 50.0 57.9 58.0 1550-1580 bp 1.0 82.1
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA TAGGCCAGACCAACCTACAA 50.0 50.0 57.9 58.0 1552-1582 bp 1.0 81.9
trnT-GGU-psbD_p3 TGAGCCCTTTTAACTCAGCG GGCCAGACCAACCTACAAAA 50.0 50.0 58.2 58.0 1553-1583 bp 1.0 81.3
trnT-GGU-psbD_p4 TGAGCCCTTTTAACTCAGCG TAGGCCAGACCAACCTACAA 50.0 50.0 58.2 58.0 1555-1585 bp 1.0 81.2
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GGCCAGACCAACCTACAAAA 50.0 50.0 58.3 58.0 1513-1543 bp 1.0 80.7