Hotspot

petN-psbM

LSC · 28549-29857 · 1309 bp

Back to Sibbaldianthe

Pi 0.00433

MarkerSeek score 58.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1309
variable_sites: 10
pi: 0.00433
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	AAAACAGTCAGCCAAGGTGA	50.0	45.0	57.6	57.6	1355-1365 bp	1.0	74.6
petN-psbM_p2	CCTGGGCTGCTTTAATGGTA	AAAACAGTCAGCCAAGGTGA	50.0	45.0	57.6	57.6	1404-1414 bp	1.0	73.9
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	AAACAGTCAGCCAAGGTGAT	50.0	45.0	57.6	57.3	1354-1364 bp	1.0	72.8
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	AACAGTCAGCCAAGGTGATT	50.0	45.0	57.6	57.3	1353-1363 bp	1.0	72.8
petN-psbM_p5	CCTGGGCTGCTTTAATGGTA	AACAGTCAGCCAAGGTGATT	50.0	45.0	57.6	57.3	1402-1412 bp	1.0	72.1