Hotspot

atpH-atpI

LSC · 13575-14043 · 469 bp

Back to Sibbaldianthe

Pi 0.00355

MarkerSeek score 63.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 469
variable_sites: 3
pi: 0.00355
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	AGGCGAATCAATGGAAGGTC	45.0	50.0	58.0	58.0	524-529 bp	1.0	83.1
atpH-atpI_p2	AATAGAAGCAAGCCCGACAG	AGGCGAATCAATGGAAGGTC	50.0	50.0	58.0	58.0	558-563 bp	1.0	83.0
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	AGGCGAATCAATGGAAGGTC	45.0	50.0	57.9	58.0	540-545 bp	1.0	82.1
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	AGGCGAATCAATGGAAGGTC	45.0	50.0	58.2	58.0	525-530 bp	1.0	82.0
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	AGGCGAATCAATGGAAGGTC	50.0	50.0	57.8	58.0	539-544 bp	1.0	81.6