Hotspot

rps16-trnQ-UUG

LSC · 6152-7150 · 999 bp

Back to Sibbaldianthe

Pi 0.017
MarkerSeek score 65.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
999
variable_sites
27
pi
0.017
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1053-1091 bp 1.0 84.5
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1051-1089 bp 1.0 84.5
rps16-trnQ-UUG_p3 GATGTAAGACTCCACAGCGG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 1067-1105 bp 1.0 84.1
rps16-trnQ-UUG_p4 GGATGTAAGACTCCACAGCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 1068-1106 bp 1.0 84.1
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1035-1073 bp 1.0 83.7