Hotspot

trnK-UUU-rps16

LSC · 4330-5007 · 678 bp

Back to Sibbaldianthe

Pi 0.015
MarkerSeek score 67.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
678
variable_sites
16
pi
0.015
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT ATTTCAAAAAGGGCGGGAGT 50.0 45.0 58.2 58.0 775-790 bp 1.0 80.3
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT ATTTCAAAAAGGGCGGGAGT 50.0 45.0 58.2 58.0 774-789 bp 1.0 80.3
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TCAAAAAGGGCGGGAGTTTT 50.0 45.0 58.2 58.2 771-786 bp 1.0 78.7
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTCAAAAAGGGCGGGAGTTT 50.0 45.0 58.2 58.2 772-787 bp 1.0 78.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTTCAAAAAGGGCGGGAGTT 50.0 45.0 58.2 58.2 773-788 bp 1.0 78.7