Hotspot

rps15-ycf1

SSC · 122913-123309 · 397 bp

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Pi 0.0176

MarkerSeek score 53.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 397
variable_sites: 14
pi: 0.0176
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	GGCAGTCGTTTCAGTATGGT	45.0	50.0	58.1	57.9	905-917 bp	0.75	70.0
rps15-ycf1_p2	CGTTGGCGTTTTCCCAAAAT	GGCAGTCGTTTCAGTATGGT	45.0	50.0	58.1	57.9	639-650 bp	0.75	69.7
rps15-ycf1_p3	CAGCTGTTCCGGTCCATTTA	GGCAGTCGTTTCAGTATGGT	50.0	50.0	57.9	57.9	1097-1109 bp	0.75	69.4
rps15-ycf1_p4	AGCTGTTCCGGTCCATTTAC	GGCAGTCGTTTCAGTATGGT	50.0	50.0	57.9	57.9	1096-1108 bp	0.75	69.4
rps15-ycf1_p5	GAGTCGAAGAACACCATGCA	GGCAGTCGTTTCAGTATGGT	50.0	50.0	58.2	57.9	914-926 bp	0.75	68.8