Hotspot

ccsA-ndhD

SSC · 114606-114871 · 266 bp

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Pi 0.0327

MarkerSeek score 61.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 266
variable_sites: 17
pi: 0.0327
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATAACGGCGCCAAGTGTTAT	GTGATACAGGGCTCAATGGG	45.0	55.0	58.0	58.0	1464-1465 bp	1.0	80.9
ccsA-ndhD_p2	CGGCGCCAAGTGTTATTTTT	GTGATACAGGGCTCAATGGG	45.0	55.0	57.9	58.0	1460-1461 bp	1.0	80.7
ccsA-ndhD_p3	ATAACGGCGCCAAGTGTTAT	CATTATCCGCTGACAGGGTC	45.0	55.0	58.0	58.1	984-985 bp	1.0	80.7
ccsA-ndhD_p4	CGGCGCCAAGTGTTATTTTT	CATTATCCGCTGACAGGGTC	45.0	55.0	57.9	58.1	980-981 bp	1.0	80.5
ccsA-ndhD_p5	ATAACGGCGCCAAGTGTTAT	TTATCTCGACGAAATGGGCG	45.0	50.0	58.0	58.2	1358-1359 bp	1.0	79.9