Hotspot

rpl22-rps19

LSC · 83857-83953 · 97 bp

Back to Sibbaldia

Pi 0.0464
MarkerSeek score 73.20
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
97
variable_sites
9
pi
0.0464
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 GGCTTTCTGTTCTTTTTCTCCA CGACTCTAAATTTCCGGGGG 40.9 55.0 57.1 58.1 172-173 bp 1.0 60.9
rpl22-rps19_p2 GGCTTTCTGTTCTTTTTCTCCA TCTAAATTTCCGGGGGCATG 40.9 50.0 57.1 57.9 168-169 bp 1.0 60.9
rpl22-rps19_p3 GGCTTTCTGTTCTTTTTCTCCA CCGACTCTAAATTTCCGGGG 40.9 55.0 57.1 58.1 173-174 bp 1.0 60.8
rpl22-rps19_p4 TGGCTTTCTGTTCTTTTTCTCC CCGACTCTAAATTTCCGGGG 40.9 55.0 57.1 58.1 174-175 bp 1.0 60.8
rpl22-rps19_p5 TGGCTTTCTGTTCTTTTTCTCC CGACTCTAAATTTCCGGGGG 40.9 55.0 57.1 58.1 173-174 bp 1.0 60.8