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psbE-petL

LSC · 64264-65519 · 1256 bp

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Pi 0.0135

MarkerSeek score 60.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1256
variable_sites: 31
pi: 0.0135
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1289-1325 bp	1.0	69.3
psbE-petL_p2	ACGAATAACCAACCCGCAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1375-1411 bp	1.0	69.0
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	1284-1320 bp	1.0	69.0
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.2	56.3	1380-1416 bp	1.0	68.8
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.4	56.3	1282-1318 bp	1.0	67.8