Hotspot

atpB-rbcL

LSC · 54447-55188 · 742 bp

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Pi 0.00472

MarkerSeek score 62.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 7
pi: 0.00472
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCCGGGTGGAAAAGATACAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	905 bp	1.0	72.1
atpB-rbcL_p2	GCCGGGTGGAAAAGATACAT	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.9	58.1	904 bp	1.0	71.8
atpB-rbcL_p3	CTTGCCGGGTGGAAAAGATA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	908 bp	1.0	71.5
atpB-rbcL_p4	AAACCCCAGGACCAGAAGTA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	828 bp	1.0	71.5
atpB-rbcL_p5	CTTGCCGGGTGGAAAAGATA	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.9	58.1	907 bp	1.0	71.2