Hotspot

ndhC-trnV-UAC

LSC · 50860-51452 · 593 bp

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Pi 0.0187

MarkerSeek score 65.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 18
pi: 0.0187
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CATATTCGTAAAGCAGCAGCA	GCACGTTGGGTCTTTGAAAC	42.9	50.0	57.0	58.2	678-759 bp	1.0	69.1
ndhC-trnV-UAC_p2	CATATTCGTAAAGCAGCAGCA	AGAAGGTCTACGGTTCGAGT	42.9	50.0	57.0	57.8	614-695 bp	1.0	69.0
ndhC-trnV-UAC_p3	CATATTCGTAAAGCAGCAGCA	TGGGTCTTTGAAACGGTTCA	42.9	45.0	57.0	57.6	672-753 bp	1.0	67.1
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGCAGC	GCACGTTGGGTCTTTGAAAC	42.9	50.0	56.8	58.2	679-760 bp	1.0	66.9
ndhC-trnV-UAC_p5	TCATATTCGTAAAGCAGCAGC	AGAAGGTCTACGGTTCGAGT	42.9	50.0	56.8	57.8	615-696 bp	1.0	66.9