Hotspot

ycf3-trnS-GGA

LSC · 44187-45041 · 855 bp

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Pi 0.00805
MarkerSeek score 59.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
855
variable_sites
11
pi
0.00805
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 557-1057 bp 1.0 71.1
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 558-1058 bp 1.0 71.1
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 507-1007 bp 1.0 70.8
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 508-1008 bp 1.0 70.8
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA AGTTCCAATGCTACGCCTTC 45.0 50.0 56.4 58.3 509-1009 bp 1.0 70.2