Hotspot

trnG-GCC-trnfM-CAU

LSC · 36146-36302 · 157 bp

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Pi 0.0385
MarkerSeek score 74.60
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
157
variable_sites
10
pi
0.0385
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 210-228 bp 1.0 86.9
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 211-229 bp 1.0 86.8
trnG-GCC-trnfM-CAU_p3 ATGCGGATATGGTCGAATGG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.9 58.0 251-269 bp 1.0 86.0
trnG-GCC-trnfM-CAU_p4 AGAAGATGCGGGTTCGATTC TGAGGTCACGGGTTCAAATC 50.0 50.0 58.1 57.8 208-226 bp 1.0 85.8
trnG-GCC-trnfM-CAU_p5 GAGAAGATGCGGGTTCGATT TGAGGTCACGGGTTCAAATC 50.0 50.0 58.1 57.8 209-227 bp 1.0 85.7