Hotspot

rpoB-trnC-GCA

LSC · 25914-27138 · 1225 bp

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Pi 0.0138

MarkerSeek score 65.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1225
variable_sites: 30
pi: 0.0138
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TAAGCCCCGATCAATGAACC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1360-1380 bp	1.0	80.0
rpoB-trnC-GCA_p2	GCCCCGATCAATGAACCTAC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.4	58.1	1357-1377 bp	1.0	79.5
rpoB-trnC-GCA_p3	TTCCTCTGTTAAGCCCCGAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1369-1389 bp	1.0	79.3
rpoB-trnC-GCA_p4	TATTGCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1294-1314 bp	1.0	79.3
rpoB-trnC-GCA_p5	AGCCCCGATCAATGAACCTA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.5	58.1	1358-1378 bp	1.0	78.8