Hotspot

trnK-UUU-rps16

LSC · 4272-4996 · 725 bp

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Pi 0.0166

MarkerSeek score 60.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 23
pi: 0.0166
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	899-952 bp	1.0	86.0
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	CGACGAGAGGGAAGAGATCT	55.0	55.0	58.0	58.0	993-1046 bp	1.0	85.7
trnK-UUU-rps16_p3	TCAGGATCAGTCGTGGTCTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.1	58.0	895-948 bp	1.0	85.5
trnK-UUU-rps16_p4	TTCAGGATCAGTCGTGGTCT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.1	58.0	896-949 bp	1.0	85.5
trnK-UUU-rps16_p5	TCAGGATCAGTCGTGGTCTT	CGACGAGAGGGAAGAGATCT	50.0	55.0	58.1	58.0	989-1042 bp	1.0	85.1