Hotspot

ndhC

LSC · 47053-47415 · 363 bp

Back to Shivparvatia

Pi 0.407

MarkerSeek score 64.10

Primer available yes

Label ndhC

Feature Metadata

label_name: ndhC
feature_type: gene
region: LSC
length_bp: 363
variable_sites: 24
pi: 0.407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.16
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC_p1	CGGAATTTTGGGTTATTCGACC	ACATTCGTAAGGAGTCTGTCG	45.5	47.6	58.0	57.5	498 bp	0.5	31.9
ndhC_p2	CGGAATTTTGGGTTATTCGACC	CCACATTCGTAAGGAGTCTGT	45.5	47.6	58.0	57.4	500 bp	0.5	31.1
ndhC_p3	CGGAATTTTGGGTTATTCGACC	TCCACATTCGTAAGGAGTCTG	45.5	47.6	58.0	57.1	501 bp	0.5	28.9
ndhC_p4	ACGGAATTTTGGGTTATTCGAC	ACATTCGTAAGGAGTCTGTCG	40.9	47.6	57.3	57.5	499 bp	0.5	26.6
ndhC_p5	CGGAATTTTGGGTTATTCGACC	TTCCACATTCGTAAGGAGTCTG	45.5	45.5	58.0	57.8	502 bp	0.5	26.3