Hotspot

ycf3-trnS-GGA

LSC · 42007-42250 · 244 bp

Back to Shivparvatia

Pi 0.0972

MarkerSeek score 81.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 244
variable_sites: 21
pi: 0.0972
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCTTTTTCTCCCGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	368-387 bp	1.0	85.4
ycf3-trnS-GGA_p2	GCCTCTTTTTCTCCCGAAGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	367-386 bp	1.0	85.4
ycf3-trnS-GGA_p3	TGCCTCTTTTTCTCCCGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	418-437 bp	1.0	85.2
ycf3-trnS-GGA_p4	GCCTCTTTTTCTCCCGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	417-436 bp	1.0	85.2
ycf3-trnS-GGA_p5	TGCCTCTTTTTCTCCCGAAG	AGTTCCAATGCTACGCCTTG	50.0	50.0	57.8	58.5	370-389 bp	1.0	82.6