Hotspot

trnS-GCU-trnS-CGA

LSC · 7856-8601 · 746 bp

Back to Shivparvatia

Pi 0.0891

MarkerSeek score 76.90

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 49
pi: 0.0891
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CTTTCGTCCACTCAGCCATC	ACCACTAAACTATACCCGCT	55.0	45.0	58.6	55.6	614-791 bp	1.0	65.7
trnS-GCU-trnS-CGA_p2	TTTCGTCCACTCAGCCATCT	ACCACTAAACTATACCCGCT	50.0	45.0	59.0	55.6	613-790 bp	1.0	62.6
trnS-GCU-trnS-CGA_p3	TCCACTCAGCCATCTCTCC	ACCACTAAACTATACCCGCT	57.9	45.0	58.1	55.6	608-785 bp	1.0	62.1
trnS-GCU-trnS-CGA_p4	GATTAGCAATCCGACGCTTTC	ACCACTAAACTATACCCGCT	47.6	45.0	57.8	55.6	630-807 bp	1.0	60.7
trnS-GCU-trnS-CGA_p5	TTCGTCCACTCAGCCATCT	ACCACTAAACTATACCCGCT	52.6	45.0	58.3	55.6	612-789 bp	1.0	60.1