Hotspot

trnK-UUU-rps16

LSC · 4116-4752 · 637 bp

Back to Shivparvatia

Pi 0.0772
MarkerSeek score 80.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
637
variable_sites
47
pi
0.0772
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA AAAGAGGGCGGCAGTTTTTA 50.0 45.0 58.0 58.0 774 bp 0.5 59.6
trnK-UUU-rps16_p2 CGAATCCCTTGCTTCATCCA AAGAGGGCGGCAGTTTTTAA 50.0 45.0 58.0 58.0 773 bp 0.5 59.6
trnK-UUU-rps16_p3 CGAATCCCTTGCTTCATCCA AGAGGGCGGCAGTTTTTAAA 50.0 45.0 58.0 58.0 772 bp 0.5 59.6
trnK-UUU-rps16_p4 CGAATCCCTTGCTTCATCCA AGGGCGGCAGTTTTTAAAGA 50.0 45.0 58.0 58.0 770 bp 0.5 59.6
trnK-UUU-rps16_p5 CGAATCCCTTGCTTCATCCA TAAAGAGGGCGGCAGTTTTT 50.0 45.0 58.0 58.0 775 bp 0.5 59.6