primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGAATCCCTTGCTTCATCCA	AAAGAGGGCGGCAGTTTTTA	20	20	50.0	45.0	58.0	58.0	0.0	12.5	0.052980	4116	4752	774-774	0.500000	59.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGAATCCCTTGCTTCATCCA	AAGAGGGCGGCAGTTTTTAA	20	20	50.0	45.0	58.0	58.0	0.0	12.5	0.052980	4116	4752	773-773	0.500000	59.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CGAATCCCTTGCTTCATCCA	AGAGGGCGGCAGTTTTTAAA	20	20	50.0	45.0	58.0	58.0	0.0	12.5	0.052980	4116	4752	772-772	0.500000	59.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CGAATCCCTTGCTTCATCCA	AGGGCGGCAGTTTTTAAAGA	20	20	50.0	45.0	58.0	58.0	0.0	12.5	0.052980	4116	4752	770-770	0.500000	59.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CGAATCCCTTGCTTCATCCA	TAAAGAGGGCGGCAGTTTTT	20	20	50.0	45.0	58.0	58.0	0.0	12.5	0.052980	4116	4752	775-775	0.500000	59.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CCACAACGGATCATGTCCTT	TTCGGAGGTTCGAATCCTTC	20	20	50.0	50.0	57.9	57.3	11.1	16.9	0.781334	5853	6537	722-783	1.000000	82.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	5853	6537	701-762	1.000000	82.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TCATGTCCTTCAAGTCGCAC	TTCGGAGGTTCGAATCCTTC	20	20	50.0	50.0	58.2	57.3	0.0	16.9	0.871706	5853	6537	712-773	1.000000	81.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TGTCGAGTCAAGAGCATCCT	TTCGGAGGTTCGAATCCTTC	20	20	50.0	50.0	58.4	57.3	0.0	16.9	1.117146	5853	6537	768-829	1.000000	79.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ATGTCGAGTCAAGAGCATCC	TTCGGAGGTTCGAATCCTTC	20	20	50.0	50.0	57.4	57.3	0.0	16.9	1.260819	5853	6537	769-830	1.000000	78.2
trnS-GCU-trnS-CGA_p1	trnS-GCU-trnS-CGA	1	CTTTCGTCCACTCAGCCATC	ACCACTAAACTATACCCGCT	20	20	55.0	45.0	58.6	55.6	0.0	0.0	2.990412	7856	8601	614-791	1.000000	65.7
trnS-GCU-trnS-CGA_p2	trnS-GCU-trnS-CGA	2	TTTCGTCCACTCAGCCATCT	ACCACTAAACTATACCCGCT	20	20	50.0	45.0	59.0	55.6	0.0	0.0	3.377515	7856	8601	613-790	1.000000	62.6
trnS-GCU-trnS-CGA_p3	trnS-GCU-trnS-CGA	3	TCCACTCAGCCATCTCTCC	ACCACTAAACTATACCCGCT	19	20	57.9	45.0	58.1	55.6	0.0	0.0	3.446452	7856	8601	608-785	1.000000	62.1
trnS-GCU-trnS-CGA_p4	trnS-GCU-trnS-CGA	4	GATTAGCAATCCGACGCTTTC	ACCACTAAACTATACCCGCT	21	20	47.6	45.0	57.8	55.6	0.0	0.0	3.594498	7856	8601	630-807	1.000000	60.7
trnS-GCU-trnS-CGA_p5	trnS-GCU-trnS-CGA	5	TTCGTCCACTCAGCCATCT	ACCACTAAACTATACCCGCT	19	20	52.6	45.0	58.3	55.6	0.0	0.0	3.683010	7856	8601	612-789	1.000000	60.1
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	TCAAGAACAGATGGAACGCT	21	20	47.6	45.0	57.3	57.2	0.0	0.0	2.532277	9601	9698	163-210	1.000000	64.0
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	TGGAACGCTTTCTACTTCAGG	21	21	47.6	47.6	57.3	58.0	0.0	2.7	2.732938	9601	9698	152-199	1.000000	62.7
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	TCAAGAACAGATGGAACGCTT	21	21	47.6	42.9	57.3	57.9	0.0	0.0	2.818792	9601	9698	163-210	1.000000	61.7
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	TTCAAGAACAGATGGAACGCT	21	21	47.6	42.9	57.3	57.9	0.0	0.0	2.818792	9601	9698	164-211	1.000000	61.7
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TGGATAGGACAGAGGTCTTCT	TCAAGAACAGATGGAACGCT	21	20	47.6	45.0	56.9	57.2	0.0	0.0	2.891090	9601	9698	198-245	1.000000	60.5
atpF-atpH_p1	atpF-atpH	1	CCAGTGACCCAAGAAAACGA	CCGAGGGAAAAATACGAGGT	20	20	50.0	50.0	58.0	57.3	0.0	0.0	0.724056	12557	13040	604-639	1.000000	82.3
atpF-atpH_p2	atpF-atpH	2	CCAGTGACCCAAGAAAACGA	GCCGAGGGAAAAATACGAGG	20	20	50.0	55.0	58.0	58.7	0.0	0.0	0.741479	12557	13040	605-640	1.000000	82.2
atpF-atpH_p3	atpF-atpH	3	CCAGTGACCCAAGAAAACGA	TGGTTGTAGCATTAGCCCTT	20	20	50.0	45.0	58.0	56.8	0.0	0.0	1.232160	12557	13040	535-570	1.000000	78.8
atpF-atpH_p4	atpF-atpH	4	CCAGTGACCCAAGAAAACGA	TTGGTTGTAGCATTAGCCCT	20	20	50.0	45.0	58.0	56.8	0.0	0.0	1.232160	12557	13040	536-571	1.000000	78.8
atpF-atpH_p5	atpF-atpH	5	CCAGTGACCCAAGAAAACGA	TGGTTGTAGCATTAGCCCTTT	20	21	50.0	42.9	58.0	57.6	0.0	0.0	1.487953	12557	13040	535-570	1.000000	76.7
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TGCCTCTTTTTCTCCCGAAG	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	57.8	57.8	0.0	5.6	0.377668	42007	42250	368-387	1.000000	85.4
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	GCCTCTTTTTCTCCCGAAGT	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	57.8	57.8	0.0	5.6	0.379196	42007	42250	367-386	1.000000	85.4
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TGCCTCTTTTTCTCCCGAAG	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.336352	42007	42250	418-437	1.000000	85.2
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	GCCTCTTTTTCTCCCGAAGT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.337880	42007	42250	417-436	1.000000	85.2
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TGCCTCTTTTTCTCCCGAAG	AGTTCCAATGCTACGCCTTG	20	20	50.0	50.0	57.8	58.5	0.0	0.0	0.731849	42007	42250	370-389	1.000000	82.6
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	GCCCCTAAACGGCGTATTTT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.9	57.9	15.2	0.0	1.035508	43174	43351	290-291	1.000000	77.5
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	GCCCCTAAACGGCGTATTTT	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.9	57.8	15.2	0.0	1.143261	43174	43351	258-259	1.000000	77.4
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	GCCCCTAAACGGCGTATTT	CTCAGAGGTTAGAGCATCGC	19	20	52.6	55.0	58.2	57.9	15.2	0.0	1.348193	43174	43351	290-291	1.000000	75.0
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	GCCCCTAAACGGCGTATTTT	CCGCTTAGCTCAGAGGTTAG	20	20	50.0	55.0	58.9	57.5	15.2	7.6	1.403276	43174	43351	298-299	1.000000	74.4
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	GCCCCTAAACGGCGTATTTT	GAATTTCTACGAGCCCGCTT	20	20	50.0	50.0	58.9	58.3	15.2	0.0	1.249616	43174	43351	313-313	0.500000	50.0
ndhC_p1	ndhC	1	CGGAATTTTGGGTTATTCGACC	ACATTCGTAAGGAGTCTGTCG	22	21	45.5	47.6	58.0	57.5	0.0	0.0	3.512919	47053	47415	498-498	0.500000	31.9
ndhC_p2	ndhC	2	CGGAATTTTGGGTTATTCGACC	CCACATTCGTAAGGAGTCTGT	22	21	45.5	47.6	58.0	57.4	0.0	0.0	3.618342	47053	47415	500-500	0.500000	31.1
ndhC_p3	ndhC	3	CGGAATTTTGGGTTATTCGACC	TCCACATTCGTAAGGAGTCTG	22	21	45.5	47.6	58.0	57.1	0.0	0.0	3.892495	47053	47415	501-501	0.500000	28.9
ndhC_p4	ndhC	4	ACGGAATTTTGGGTTATTCGAC	ACATTCGTAAGGAGTCTGTCG	22	21	40.9	47.6	57.3	57.5	0.0	0.0	4.179365	47053	47415	499-499	0.500000	26.6
ndhC_p5	ndhC	5	CGGAATTTTGGGTTATTCGACC	TTCCACATTCGTAAGGAGTCTG	22	22	45.5	45.5	58.0	57.8	0.0	0.0	4.211985	47053	47415	502-502	0.500000	26.3
petA-psbJ_p1	petA-psbJ	1	GTTCAATTGTCCGAAATGAA	ATTCTGGATTGGGCTCATCG	20	20	35.0	50.0	52.4	57.7	5.1	0.0	5.884440	58833	59686	794-907	1.000000	48.2
petA-psbJ_p2	petA-psbJ	2	TCAATTGTCCGAAATGAATTTCT	ATTCTGGATTGGGCTCATCG	23	20	30.4	50.0	55.1	57.7	12.6	0.0	6.128554	58833	59686	792-905	1.000000	48.2
petA-psbJ_p3	petA-psbJ	3	GTTCAATTGTCCGAAATGAATTTCT	ATTCTGGATTGGGCTCATCG	25	20	32.0	50.0	57.1	57.7	12.6	0.0	6.140913	58833	59686	794-907	1.000000	48.2
petA-psbJ_p4	petA-psbJ	4	GTTCAATTGTCCGAAATGAAT	ATTCTGGATTGGGCTCATCG	21	20	33.3	50.0	53.1	57.7	5.1	0.0	6.187536	58833	59686	794-907	1.000000	48.2
petA-psbJ_p5	petA-psbJ	5	GTTCAATTGTCCGAAATGAATT	ATTCTGGATTGGGCTCATCG	22	20	31.8	50.0	53.9	57.7	5.1	0.0	6.330840	58833	59686	794-907	1.000000	48.2
clpP_p1	clpP	1	CGTTAACGCTACCAATGGGA	GCCGGGATCATTCAATACGA	20	20	50.0	50.0	58.0	57.8	16.0	2.2	0.176755	65747	67769	2920-2928	1.000000	84.0
clpP_p2	clpP	2	CTAGTACATGTTCCTCGGCG	ACGAACATACCCTGTCTCCA	20	20	55.0	50.0	57.9	58.1	2.3	0.0	0.188095	65747	67769	2857-2902	1.000000	83.6
clpP_p3	clpP	3	CTAGTACATGTTCCTCGGCG	TCGGATCGGTTATAGTCCCC	20	20	55.0	55.0	57.9	58.1	2.3	0.0	0.205975	65747	67769	2937-2982	1.000000	83.4
clpP_p4	clpP	4	TGACCCTGTTAGTCCGTTCT	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.242663	65747	67769	2970-2978	1.000000	83.4
clpP_p5	clpP	5	CGTTAACGCTACCAATGGGA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	16.0	0.0	0.250229	65747	67769	2889-2897	1.000000	83.4
rpl16_p1	rpl16	1	AGCATATCGGCGATTGCTAG	CAAGAACATCCTCGGGTGTC	20	20	50.0	55.0	58.0	58.3	35.6	1.7	0.274959	76783	78134	2165-2211	1.000000	83.9
rpl16_p2	rpl16	2	CATATCGGCGATTGCTAGCT	CAAGAACATCCTCGGGTGTC	20	20	50.0	55.0	58.0	58.3	28.9	1.7	0.274959	76783	78134	2163-2209	1.000000	83.9
rpl16_p3	rpl16	3	GAGCATATCGGCGATTGCTA	CAAGAACATCCTCGGGTGTC	20	20	50.0	55.0	58.0	58.3	36.0	1.7	0.276855	76783	78134	2166-2212	1.000000	83.9
rpl16_p4	rpl16	4	TTTTCTCAATTCCCGGGCAA	CAAGAACATCCTCGGGTGTC	20	20	45.0	55.0	58.0	58.3	18.5	1.7	0.275652	76783	78134	2390-2436	1.000000	83.6
rpl16_p5	rpl16	5	TTTTTCTCAATTCCCGGGCA	CAAGAACATCCTCGGGTGTC	20	20	45.0	55.0	58.0	58.3	18.5	1.7	0.275652	76783	78134	2391-2437	1.000000	83.6
ndhF_p1	ndhF	1	GGAATCGTCCAACGCGATAT	ACTAAAAGATGCAACCCGAA	20	20	50.0	40.0	58.2	55.3	15.9	0.0	2.955022	104947	107178	2958-2958	0.500000	36.4
ndhF_p2	ndhF	2	GGAATCGTCCAACGCGATAT	ACTAAAAGATGCAACCCGAAA	20	21	50.0	38.1	58.2	56.1	15.9	0.0	3.148354	104947	107178	2958-2958	0.500000	34.8
ndhF_p3	ndhF	3	GGAATCGTCCAACGCGATAT	ACTAAAAGATGCAACCCGAAAT	20	22	50.0	36.4	58.2	56.6	15.9	0.0	3.624402	104947	107178	2958-2958	0.500000	31.0
ndhF_p4	ndhF	4	AAAAAGGCTTTTCACCCTCT	ACATACTAAACCACGCCGAT	20	20	40.0	45.0	55.3	57.0	24.3	0.0	3.644798	104947	107178	2942-2942	0.500000	30.8
ndhF_p5	ndhF	5	AAAAAGGCTTTTCACCCTCT	TGTACATACTAAACCACGCCG	20	21	40.0	47.6	55.3	58.1	24.3	0.0	3.792524	104947	107178	2945-2945	0.500000	29.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ACGAAAAATAAGTACTCAAAACTTGG	TCCTAAGAGCAGCGTGTCTA	26	20	30.8	50.0	56.9	57.9	0.0	0.0	7.198067	108098	108797	718-770	1.000000	50.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ACGAAAAATAAGTACTCAAAACTTGGA	TCCTAAGAGCAGCGTGTCTA	27	20	29.6	50.0	58.1	57.9	0.0	0.0	7.228540	108098	108797	718-770	1.000000	50.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ACGAAAAATAAGTACTCAAAACTTGG	AGCAGCGTGTCTACCAATTT	26	20	30.8	45.0	56.9	57.8	0.0	0.0	7.258794	108098	108797	711-763	1.000000	50.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ACGAAAAATAAGTACTCAAAACTTGG	CCTAAGAGCAGCGTGTCTAC	26	20	30.8	55.0	56.9	57.8	0.0	0.0	7.270084	108098	108797	717-769	1.000000	50.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACGAAAAATAAGTACTCAAAACTTGG	CTAAGAGCAGCGTGTCTACC	26	20	30.8	55.0	56.9	57.8	0.0	0.0	7.270084	108098	108797	716-768	1.000000	50.6
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TAGACACGCTGCTCTTAGGA	TGAGTCAAAATATGTTCTAAAGTCGA	20	26	50.0	30.8	57.9	56.9	0.0	0.0	7.175539	108878	109016	225-235	1.000000	47.3
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	GTAGACACGCTGCTCTTAGG	TGAGTCAAAATATGTTCTAAAGTCGA	20	26	55.0	30.8	57.8	56.9	0.0	0.0	7.247557	108878	109016	226-236	1.000000	47.3
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GGTAGACACGCTGCTCTTAG	TGAGTCAAAATATGTTCTAAAGTCGA	20	26	55.0	30.8	57.8	56.9	0.0	0.0	7.247557	108878	109016	227-237	1.000000	47.2
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	AAATTGGTAGACACGCTGCT	TGAGTCAAAATATGTTCTAAAGTCGA	20	26	45.0	30.8	57.8	56.9	0.0	0.0	7.236266	108878	109016	232-242	1.000000	47.1
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	AATTGGTAGACACGCTGCTC	TGAGTCAAAATATGTTCTAAAGTCGA	20	26	50.0	30.8	58.3	56.9	0.0	0.0	7.324408	108878	109016	231-241	1.000000	47.1