Hotspot

ycf3

LSC · 44453-46439 · 1987 bp

Back to Shibataea

Pi 0.000336
MarkerSeek score 45.50
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
1987
variable_sites
1
pi
0.000336
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 ATCAACAACCAAACCACCCA AGAGCATATGGATCGATCGA 45.0 45.0 57.8 55.7 2130-2131 bp 1.0 60.4
ycf3_p2 TCAACAACCAAACCACCCAT AGAGCATATGGATCGATCGA 45.0 45.0 57.8 55.7 2129-2130 bp 1.0 60.4
ycf3_p3 ATCAACAACCAAACCACCCA AGAGCATATGGATCGATCGAT 45.0 42.9 57.8 56.3 2130-2131 bp 1.0 56.9
ycf3_p4 TCAACAACCAAACCACCCAT AGAGCATATGGATCGATCGAT 45.0 42.9 57.8 56.3 2129-2130 bp 1.0 56.9
ycf3_p5 GATCAACAACCAAACCACCC AGAGCATATGGATCGATCGA 50.0 45.0 57.2 55.7 2131-2132 bp 1.0 55.3