Hotspot

psaA

LSC · 41572-43824 · 2253 bp

Back to Shibataea

Pi 0.000296
MarkerSeek score 44.90
Primer available yes
Label psaA

Feature Metadata

label_name
psaA
feature_type
gene
region
LSC
length_bp
2253
variable_sites
1
pi
0.000296
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA_p1 AGCTAAGCCTTGGCTAAACC TTGGCGGGTCTCTTTGTATG 50.0 50.0 57.9 57.9 2362 bp 1.0 78.1
psaA_p2 GCTAAGCCTTGGCTAAACCT TTGGCGGGTCTCTTTGTATG 50.0 50.0 57.9 57.9 2361 bp 1.0 78.1
psaA_p3 AGCTAAGCCTTGGCTAAACC GTTGGCGGGTCTCTTTGTAT 50.0 50.0 57.9 57.9 2363 bp 1.0 78.1
psaA_p4 GCTAAGCCTTGGCTAAACCT GTTGGCGGGTCTCTTTGTAT 50.0 50.0 57.9 57.9 2362 bp 1.0 78.1
psaA_p5 AATACGACGAGTAGTGGGGT TTGGCGGGTCTCTTTGTATG 50.0 50.0 57.9 57.9 2386 bp 1.0 78.1