Hotspot

infA

LSC · 78815-79207 · 393 bp

Back to Shibataea

Pi 0

MarkerSeek score 58.40

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 393
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	GTCTCTTCGCTTGAATTTTGGA	ATGGTAATCGCCCCTCCTAT	40.9	50.0	57.3	57.7	517-568 bp	1.0	59.3
infA_p2	AGTCTCTTCGCTTGAATTTTGG	ATGGTAATCGCCCCTCCTAT	40.9	50.0	57.2	57.7	518-569 bp	1.0	59.2
infA_p3	GTCTCTTCGCTTGAATTTTGGA	CGCCCCTCCTATAGTACTCG	40.9	60.0	57.3	58.5	509-560 bp	1.0	57.6
infA_p4	AGTCTCTTCGCTTGAATTTTGG	CGCCCCTCCTATAGTACTCG	40.9	60.0	57.2	58.5	510-561 bp	1.0	57.6
infA_p5	TCTCTTCGCTTGAATTTTGGAAC	ATGGTAATCGCCCCTCCTAT	39.1	50.0	57.9	57.7	516-567 bp	1.0	56.4