Hotspot

trnR-ACG-rrn5

IRa · 120448-120705 · 258 bp

Back to Shibataea

Pi 0
MarkerSeek score 30.00
Primer available yes
Label trnR-ACG-rrn5

Feature Metadata

label_name
trnR-ACG-rrn5
feature_type
igs
region
IRa
length_bp
258
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5_p1 CTCAGGGGGAAAGTAAAGGC CACACCAATCCATCCCGAAT 55.0 50.0 57.9 57.9 466 bp 1.0 78.5
trnR-ACG-rrn5_p2 CTCAGGGGGAAAGTAAAGGC GGTGACGATACTGTAGGGGA 55.0 55.0 57.9 57.9 425 bp 1.0 78.5
trnR-ACG-rrn5_p3 CTCAGGGGGAAAGTAAAGGC ATCCATCCCGAATTTGGTGG 55.0 50.0 57.9 57.9 459 bp 1.0 78.4
trnR-ACG-rrn5_p4 GAAAGTAAAGGCCCTTCCCC CACACCAATCCATCCCGAAT 55.0 50.0 58.2 57.9 458 bp 1.0 78.3
trnR-ACG-rrn5_p5 GGAAAGTAAAGGCCCTTCCC CACACCAATCCATCCCGAAT 55.0 50.0 58.2 57.9 459 bp 1.0 78.3