Hotspot

rps15-trnN-GUU

IRa · 118504-120044 · 1541 bp

Back to Shibataea

Pi 0.000433
MarkerSeek score 46.40
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
IRa
length_bp
1541
variable_sites
1
pi
0.000433
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 CAGAACCCCTGTTTTCTTCT ACGACCAGTCAGTTAACAGC 45.0 50.0 55.2 57.8 1663 bp 1.0 56.2
rps15-trnN-GUU_p2 CAGAACCCCTGTTTTCTTCT AGGATTCGAACCTACGACCA 45.0 50.0 55.2 58.2 1676 bp 1.0 56.1
rps15-trnN-GUU_p3 CAGAACCCCTGTTTTCTTCT GGATTCGAACCTACGACCAG 45.0 55.0 55.2 57.8 1675 bp 1.0 55.8
rps15-trnN-GUU_p4 ACTTGAAATTCAACAGAACCCC ACGACCAGTCAGTTAACAGC 40.9 50.0 57.1 57.8 1676 bp 1.0 55.3
rps15-trnN-GUU_p5 ACTTGAAATTCAACAGAACCCC AGGATTCGAACCTACGACCA 40.9 50.0 57.1 58.2 1689 bp 1.0 55.3