Hotspot

ndhC-trnV-UAC

LSC · 52501-53274 · 774 bp

Back to Shibataea

Pi 0
MarkerSeek score 50.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
774
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTCACTAATCGGGGCTA GGTTCGAATCCGTATAGCCC 50.0 55.0 57.5 57.9 880-907 bp 1.0 78.1
ndhC-trnV-UAC_p2 CTGGTCCTTCACTAATCGGG GGTTCGAATCCGTATAGCCC 55.0 55.0 57.4 57.9 884-911 bp 1.0 77.0
ndhC-trnV-UAC_p3 TCCTTCACTAATCGGGGCTA CGGTTCGAATCCGTATAGCC 50.0 55.0 57.5 58.3 881-908 bp 1.0 76.2
ndhC-trnV-UAC_p4 TGGTCCTTCACTAATCGGGG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.8 57.9 883-910 bp 1.0 75.4
ndhC-trnV-UAC_p5 CTGGTCCTTCACTAATCGGG CGGTTCGAATCCGTATAGCC 55.0 55.0 57.4 58.3 885-912 bp 1.0 75.1