Hotspot

ndhA

SSC · 113522-115628 · 2107 bp

Back to Setaria

Pi 0.00775

MarkerSeek score 61.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2107
variable_sites: 29
pi: 0.00775
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAACCCAGTTAGCATAGGGA	TATGGGGGAAGTTGACCGTT	47.6	50.0	58.2	58.6	2232-2279 bp	1.0	67.6
ndhA_p2	TGAACCCAGTTAGCATAGGGA	TTATGGGGGAAGTTGACCGT	47.6	50.0	58.2	58.6	2233-2280 bp	1.0	67.6
ndhA_p3	TGAACCCAGTTAGCATAGGGA	ATTATGGGGGAAGTTGACCG	47.6	50.0	58.2	57.3	2234-2281 bp	1.0	66.8
ndhA_p4	CCCAGTTAGCATAGGGAACA	TATGGGGGAAGTTGACCGTT	50.0	50.0	56.6	58.6	2228-2275 bp	1.0	65.7
ndhA_p5	CCCAGTTAGCATAGGGAACA	TTATGGGGGAAGTTGACCGT	50.0	50.0	56.6	58.6	2229-2276 bp	1.0	65.7